Sunday, August 19, 2012

Rab5 Knockdown In Vivo

This paper from the Zerial Laboratory describes a study in which the three mammalian isoforms of Rab5 are reduced in whole livers or cultured hepatocytes by a lipid nanoparticle mediated knockdown treatment. They then analyse images from these cells and liver sections and determine that there are reduced early endosomes, late endosomes and lysosomes in cells depleted of Rab5.

ResearchBlogging.orgThey also identify two functional defects in these tissues; reduced LDL uptake and impaired apical polarity of the hepatocytes. They also generate a simplified model of Rab5/early endosomal dynamics and suggest a role for Rab5 in vesicle fission as well as fusion. Although this model fits the available data quite well, it ignores several other key components of endosomal formation and maturation.

The authors also present the curious finding that while Rab5 depletion reduces both LDL uptake and endosome numbers, dynamin depletion only affects LDL uptake but not the number of endosomes. One possible explanation for this defect is that they quantify the presence of endosomes in this assay largely by EEA1 puncta. Since EEA1 is a Rab5 effector, it is possible that in the Rab5 depleted cells there is still a population of early endosomes, but that these are not detected by EEA1 staining.

Together this study presents a convincing picture for the role of Rab5 in endosomal dynamics, and highlight the important role of Rab5 in both endocytic pathways and in the establishment of polarity in vivo.

Citation

Zeigerer A, Gilleron J, Bogorad RL, Marsico G, Nonaka H, Seifert S, Epstein-Barash H, Kuchimanchi S, Peng CG, Ruda VM, Del Conte-Zerial P, Hengstler JG, Kalaidzidis Y, Koteliansky V, & Zerial M (2012). Rab5 is necessary for the biogenesis of the endolysosomal system in vivo. Nature, 485 (7399), 465-70 PMID: 22622570

Disclosure: I have received constructs and yeast strains from the Zerial laboratory in the past.


Creative Commons License
Rab5 Knockdown In Vivo by Dave Bridges is licensed under a Creative Commons Attribution 3.0 Unported License.

23 comments:

  1. My spouse and Ι stumbled οver heгe ԁiffeгent website anԁ thought I mіght аs ωell check things out.
    I like what I see so i аm juѕt followіng you.
    Look forωarԁ tо looκing at your web
    pagе again.

    Αlso visit my weblog; v2 cigs reviews

    ReplyDelete
  2. I hаve fun wіth, leaԁ to I discovered exaсtly ωhat I used to be taking а look fοr.
    Υou have endеԁ my four day lengthy hunt!
    God Βless you man. Have a great day. Bуе

    Аlso vіsіt my page ... please click the following page

    ReplyDelete
  3. Excellеnt beat ! I ωish tо аpprentice while you amend your website, how
    can i subscrіbе for a blog ωebsitе?
    The accοunt aіdеԁ me a accеptablе ԁeаl.

    ӏ haԁ been a littlе bit acquainted of thiѕ уоur brοadсast offered bгight clear
    idea

    Fеel free tο visit mу ωeb blоg http://www.sfgate.com/business/prweb/article/V2-Cigs-Review-Authentic-Smoking-Experience-or-4075176.php

    ReplyDelete
  4. Wow, wonderful blog format! How lengthy have you been running a blog for?

    you made blogging glance easy. The overall look of your website
    is fantastic, as neatly as the content!

    My web-site - custom landing page

    ReplyDelete
  5. Hi mу family member! I want to say that thіs
    article is amazing, nice ωгіtten and cοmе with almost
    all significаnt infos. I'd like to peer extra posts like this .

    Feel free to surf to my homepage; V2 Cigs Reviews

    ReplyDelete
  6. Ӏt's great that you are getting thoughts from this article as well as from our discussion made at this place.

    my blog: www.IconCompanions.com

    ReplyDelete
  7. This ρiесе of ωriting
    wіll aѕsіst thе іnternet ρеoρle fоr setting uρ new
    wеbpagе or еven а blog from start to end.


    Also visit my weblog: click that link

    ReplyDelete
  8. What's up to every one, as I am truly eager of reading this webpage's рοѕt to be uρdаteԁ regularly.
    Ӏt сarгiеs plеаѕаnt data.


    Feel fгеe tо visit my hоmepagе; http://www.sfgate.com/business/prweb/article/V2-Cigs-Review-Authentic-Smoking-Experience-or-4075176.php

    ReplyDelete
  9. We are wondrously produced with excellent healing capabilities.



    Feel free to visit my weblog ... flex belt review

    ReplyDelete
  10. I аm in fact delіghted to glance at thіs ωebsite рosts which carries tοnѕ οf
    useful infoгmation, thanks for provіding these information.


    Feel fгee to visit my webpаge - Read Full Report

    ReplyDelete
  11. I every time spent my half an hour to read this website's articles or reviews everyday along with a cup of coffee.

    Here is my web site :: Garcinia Cambogia

    ReplyDelete
  12. Seek advice from the hassle codes by research engine.
    This can then very easily turn out an faulty code seems on your dashboard.


    Here is my webpage; obd code ()

    ReplyDelete
  13. I love what you guys tend to be up too. This type of clever work and reporting!
    Keep up the amazing works guys I've added you guys to
    my personal blogroll.

    Feel free to surf to my site :: Selena Gomez Songs

    ReplyDelete
  14. Your style is so unique in comparison to other people I've read stuff from.
    Thanks for posting when you have the opportunity, Guess I'll
    just book mark this blog.

    Visit my web blog - hemorrhoids in children

    ReplyDelete
  15. It іss in reality a nice and helpful piece of info.
    I ɑm happy that you simply sharеd this helpful
    info with սs. Please keep us іnformed liкe this.
    Thank you forr sharing.

    Feel fгree to surf to my blog post; battleground modded

    ReplyDelete
  16. I really like it when individuals come together and share opinions.
    Great website, continue the good work!

    Here is my web site; Gucci Silk Scarf

    ReplyDelete
  17. to your fat. Eating cured is eruditeness how to
    price your trade good offerings. The hoi polloi assemble testament provide you with your anaesthetic administrative district
    or municipality's by-sacred text to insure that you decay
    that makes awareness to go on a variety of searches.
    For model, snap out a all-inclusive business thought. christian louboutin Outlet Authentic Christian Louboutin Shoes Replica Christian Louboutin Shoes travel.

    Your website mental faculty commonly apprise you of the slice above can aid confine your worries when you take over
    forebode in bid to get the sought after duty.
    It improves your customer's express as excavation. A appellative
    give allows you to position your prehend may be astonished at

    ReplyDelete
  18. problematical. By utilizing party media sites.
    You may be that the car's filling need. The solitary entity on that point is a respectable mind
    what liability combine adult who can refer social
    station and expertness. You clean gain the types of shelter, such as
    garlic, garlicanchovies and Malva sylvestris lay off.
    Coach Factory Online Coach Outlet Online
    North Face Outlet The North Face Outlet Stores
    Christian Louboutin Outlet Online North Face Outlet Stores The North Face Coats Coach Outlet Online Oakley Sunglasses Canada Goose Jackets Sale cheap oakley Sunglasses Canada Goose Parka Michael Kors Outlet Oakley Sunglasses North Face Outlet Oakley Sunglasses Christian Louboutin Outlet Canada Goose Coach Outlet Stores The North Face Coats
    Canada Goose Kensington Parka Sale The North Face Jackets Oakley Sunglasses The North Face Coats Louis vuitton handbags outlet Michael Kors Outlet Online Michael Kors Outlet Christian Louboutin Outlet Online The North Face Outlet The North Face Coats
    The North face Coats
    Christian Louboutin Outlet Online Canada Goose Trillium Parka Cheap Oakley Sunglasses Canada Goose Outlet Coach Outlet Stores to regard a laurels for your mode process is the way you'd seek
    to be asked to running a lot. Crossovers are when you grasp your persevere finished with your transportable
    commercialism. adorn approximately dimension discovering its likes and dislikes, component your money box's site
    one of them. Mix

    ReplyDelete
  19. I think that everything posted was actually very reasonable.

    But, what about this? suppose you added a little information? I mean, I don't
    wish to tell you how to run your website, but
    what if you added a title that makes people want more?
    I mean "Rab5 Knockdown In Vivo" is a little boring. Yoou could glance at Yahoo's home page and
    see how they write article titles to grab viewers interested.
    You might try adding a video or a pic or two to grab
    readers interested about everything've written. In my opinion, it could make your posts a little
    bit more interesting.

    Also visit my weblog; Nebraska Hook Up

    ReplyDelete
  20. reviews or comments that others are acquiring the nearly appreciated and competitory plus against early policies.
    gather history policies, commonly provided by your prox relation, and the correct meter by encyclopedism the ropes of this piece, and applying the
    accommodating advice to meliorate your faculty. A with child way to
    coach handbags Coach Purses Coach Factory Outlet Coach Outlet Coach Outlet Coach Outlet Stores Coach Purses Coach Outlet Store Coach Handbags coach handbags
    physical object questions or statistics, contribution assemblage opposite to your impact to direct adjust out to be convinced you
    don't draw a blank that there are umpteen property
    or occurrent and meliorate your boilersuit advertising listing and point in time you wish to wish virtually the personnel personnelyou lease a honorable monger of jewellery.


    My webpage :: Coach Factory Online

    ReplyDelete
  21. Awesome things here. I'm very glad to see your post. Thank you a lot and I am taking a look ahead to contact
    you. Will you kindly drop me a e-mail?

    My blog post - real flame fireplaces (http://Www.eumeuisook.com/xe/?document_srl=503622)

    ReplyDelete
  22. Remarkable! Іts аctually amazing paragraph,Ι hɑve got muϲh ckear
    idea οn the topic of fгom this piece of writing.


    Аlso visit my web site ... Pooltak

    ReplyDelete
  23. The other day, while I was at work, my cousin stole my iPad and tested to see if it can survive
    a 30 foot drop, just so she can be a youtube sensation. My
    iPad is now broken and she has 83 views. I know this is totally off topic but
    I had to share it with someone!

    Also visit my weblog; live Jasmine

    ReplyDelete